626 cd34  (Bioss)

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunohistochemical staining results of young and adult Tibetan sheep myocardium(scale bar, 10 μm). Immunohistochemical staining results of young and adult Tibetan sheep myocardium(scale bar, 10 µm).A-a: The expression of CD34 in the myocardium of young Tibetan sheep . A-b: The expression of CD117 in the myocardium of young Tibetan sheep. A-c: The expression of PDGFR-α in the myocardium of young Tibetan sheep. A-d: The expression of α-SMA in the myocardium of young Tibetan sheep. B-a: The expression of CD34 in the myocardium of adult Tibetan sheep. B-b: The expression of CD117 in the myocardium of adult Tibetan sheep. B-c: The expression of PDGFR-α in the myocardium of adult Tibetan sheep. B-d: The expression of α-SMA in the myocardium of adult Tibetan sheep. Black arrows indicate TCs

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunohistochemical staining results of young and adult Tibetan sheep heart aortic bulb(scale bar, 10 μm). A-a: The expression of CD34 in the aortic bulb of young Tibetan sheep . A-b: The expression of CD117 in the aortic bulb of young Tibetan sheep. A-c: The expression of PDGFR-α in the aortic bulb of young Tibetan sheep. A-d: The expression of α-SMA in the aortic bulb of young Tibetan sheep. B-a: The expression of CD34 in the aortic bulb of adult Tibetan sheep. B-b: The expression of CD117 in the aortic bulb of adult Tibetan sheep. B-c: The expression of PDGFR-α in the aortic bulb of adult Tibetan sheep. B-d: The expression of α-SMA in the aortic bulb of adult Tibetan sheep. Black arrows indicate TCs

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunofluorescence double staining results of young and adult Tibetan sheep myocardium (scale bar, 50 μm). A-a: The expression of CD34 in the myocardium of young Tibetan sheep. A-b: The expression of CD117 in the myocardium of young Tibetan sheep. A-c: The expression of DAPI in the myocardium of young Tibetan sheep. A-d: Co-expression of CD34 and CD117 in the myocardium of young Tibetan sheep. A-e: The expression of CD34 in the myocardium of young Tibetan sheep. A-f: The expression of α-SMA in the myocardium of young Tibetan sheep. A-g: The expression of DAPI in the myocardium of young Tibetan sheep. A-h: The co-expression of CD34 and α-SMA in the myocardium of young Tibetan sheep. A-i: The expression of PDGFR-α in the myocardium of young Tibetan sheep. A-j: The expression of CD117 in the myocardium of young Tibetan sheep. A-k: The expression of DAPI in the myocardium of young Tibetan sheep. A-l: Co-expression of PDGFR-α and CD117 in the myocardium of young Tibetan sheep. B-a: The expression of CD34 in the myocardium of adult Tibetan sheep. B-b: The expression of CD117 in the myocardium of adult Tibetan sheep. B-c: The expression of DAPI in the myocardium of adult Tibetan sheep. B-d: Co-expression of CD34 and CD117 in the myocardium of adult Tibetan sheep. B-e: The expression of CD34 in the myocardium of adult Tibetan sheep. B-f: The expression of α-SMA in the myocardium of adult Tibetan sheep. B-g: The expression of DAPI in the myocardium of adult Tibetan sheep. B-h: The co-expression of CD34 and α-SMA in the myocardium of adult Tibetan sheep. B-i: The expression of PDGFR-α in the myocardium of adult Tibetan sheep. B-j: The expression of CD117 in the myocardium of adult Tibetan sheep. B-k: The expression of DAPI in the myocardium of adult Tibetan sheep. B-l: Co-expression of PDGFR-α and CD117 in the myocardium of adult Tibetan sheep

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunofluorescence, Double Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Immunofluorescence double staining results of young and adult Tibetan sheep heart aortic bulb (scale bar, 50 μm). A-a: The expression of CD34 in the aortic bulb of young Tibetan sheep. A-b: The expression of CD117 in the aortic bulb of young Tibetan sheep. A-c: The expression of DAPI in the aortic bulb of young Tibetan sheep . A-d: Co-expression of CD34 and CD117 in the aortic bulb of young Tibetan sheep . A-e: The expression of CD34 in the aortic bulb of young Tibetan sheep. A-f: The expression of α-SMA in the aortic bulb of young Tibetan sheep. A-g: The expression of DAPI in the aortic bulb of young Tibetan sheep. A-h: The co-expression of CD34 and α-SMA in the aortic bulb of young Tibetan sheep . A-i: The expression of PDGFR-α in the aortic bulb of young Tibetan sheep. A-j: The expression of CD117 in the aortic bulb of young Tibetan sheep. A-k: The expression of DAPI in the aortic bulb of young Tibetan sheep. A-l: Co-expression of PDGFR-α and CD117 in the aortic bulb of young Tibetan sheep. B-a: The expression of CD34 in the aortic bulb of adult Tibetan sheep. B-b: The expression of CD117 in the aortic bulb of adult Tibetan sheep. B-c: The expression of DAPI in the aortic bulb of adult Tibetan sheep. B-d: Co-expression of CD34 and CD117 in the aortic bulb of adult Tibetan sheep. B-e: The expression of CD34 in the aortic bulb of adult Tibetan sheep. B-f: The expression of α-SMA in the aortic bulb of adult Tibetan sheep. B-g: The expression of DAPI in the aortic bulb of adult Tibetan sheep. B-h: The co-expression of CD34 and α-SMA in the aortic bulb of adult Tibetan sheep. B-i: The expression of PDGFR-α in the aortic bulb of adult Tibetan sheep. B-j: The expression of CD117 in the aortic bulb of adult Tibetan sheep. B-k: The expression of DAPI in the aortic bulb of adult Tibetan sheep. B-l: Co-expression of PDGFR-α and CD117 in the aortic bulb of adult Tibetan sheep.

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Immunofluorescence, Double Staining, Expressing

Journal: BMC Veterinary Research

Article Title: The ultrastructural characterization and immunophenotypic features of telocytes in the myocardium and aortic bulb of young and adult Tibetan sheep

doi: 10.1186/s12917-026-05350-w

Figure Lengend Snippet: Protein expression of effective telocyte markers (CD117, CD34, α-SMA and PDGFR-α) in the myocardium and aortic bulb of young and adult Tibetan sheep. ( a ) Western blotting, ( b ) CD117 Relative protein expression levels, ( c ) CD34 Relative protein expression levels, ( d ) α-SMA Relative protein expression levels, ( e ) PDGFR-α Relative protein expression levels. Each sample was tested three times. Results are expressed as the mean±standard deviation, with β-actin protein expression used as an internal control. *** P <0.001, ns (not significant), P >0.05

Article Snippet: Immunohistochemistry kit (SP-0023) and the rabbit polyclonal antibody CD34 (bs-8996R) and CD117 (bs-1005R) were purchased from Beijing Biosynthesis Biotechnology Co., Ltd. (Beijing, China), DAB chromogenic kit (ZLI-9018) were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. (Beijing, China), the α-SMA (AF-1032) were purchased from Jiangsu KeyGEN BioTECH Co., Ltd. (Nanjing City, China) mouse monoclonal antibody CD34 (bsm-41196 M) were purchased from Beijing Bioss Biotechnology Co., Ltd., PDGFR-α (14-14018-2) were purchased from Thermo Fisher Scientific (Watlham, MA, USA), and Goat Anti-Mouse IgG H&L (ab150113, Alexa Fluor 488) and Goat Anti-Rabbit IgG H&L (ab150077, Alexa Fluor 488; ab150079, Alexa Fluor 647) were purchased from Abcam (Cambridge, UK).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: Journal of Traditional and Complementary Medicine

Article Title: Plasma-derived exosomal miR-144-3p targeting IL-1β is involved in pressure pain threshold regulation at PC6 acupoint in myocardial ischemia rats

doi: 10.1016/j.jtcme.2025.02.001

Figure Lengend Snippet: Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of CD34 (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.

Article Snippet: The sections were incubated with primary antibodies including a rabbit polyclonal anti-CD34 antibody (Affinity Biosciences, Cincinnati, Ohio, USA) and rabbit polyclonal anti-Protein gene product 9.5 (PGP9.5) antibody (Affinity Biosciences, Cincinnati, Ohio, USA).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: NPJ Regenerative Medicine

Article Title: Deer antler ASCs exosomes ameliorate osteoarthritis via miR-140/MMP13 axis-mediated dual modulation of inflammation and cartilage regeneration

doi: 10.1038/s41536-025-00444-9

Figure Lengend Snippet: A Schematic illustration of the ASCs isolation procedure from deer antler tissue using mechanical dissection and the collagenase digestion method. Created in BioRender. Yuhao, S. (2025) https://BioRender.com/m5easfx . B Representative phase-contrast microscopy image showing the typical spindle-shaped morphology of cultured ASCs at passage 3. Scale bar: 100 μm. C Immunofluorescence analysis of ASC surface markers. ASCs were positive for CD73 and CD90 (green), while negative for CD34 and CD45. Nuclei were counterstained with DAPI (blue). Merged images show co-localization. Scale bar: 100 μm. D – F Multi-lineage differentiation potential of ASCs. D Oil Red O staining showing lipid droplets after 14 days of adipogenic induction. Scale bars: 100 μm. E Alizarin Red S staining demonstrating calcium deposition after 21 days of osteogenic differentiation. Scale bars: 500 μm. F Alcian Blue staining revealing proteoglycan synthesis after 14 days of chondrogenic induction. Scale bars: 100 μm. G Western blot analysis confirming the expression of exosomal markers (CD63, TSG101, and CD9) in isolated ASC-Exos compared to ASCs and culture supernatant. H Representative transmission electron microscopy (TEM) image showing the typical cup-shaped morphology of ASC-Exos with characteristic double-membrane structure. Scale bars: 100 nm. I Nanoparticle tracking analysis (NTA) reveals the size distribution of ASC-Exos with an average diameter of 86.4 nm. J Scatter plot from flow cytometry analysis demonstrating the uniform distribution and homogeneity of the isolated ASC-Exos population.

Article Snippet: For flow cytometric analysis, cells were dissociated using trypsin without EDTA, washed with PBS, and incubated with surface marker antibodies at room temperature for 1 h. The panel included positive markers CD73 (bs-4834R, Bioss, China) and CD90 (bs-0778R, Bioss, China), and negative hematopoietic markers CD45 (bs-4819R, Bioss, China) and CD34 (bs-0646R, Bioss, China), following the manufacturer’s recommended antibody concentrations.

Techniques: Isolation, Dissection, Microscopy, Cell Culture, Immunofluorescence, Staining, Western Blot, Expressing, Transmission Assay, Electron Microscopy, Membrane, Flow Cytometry