626 cd34  (Bioss)

Journal: NPJ Regenerative Medicine

Article Title: Deer antler ASCs exosomes ameliorate osteoarthritis via miR-140/MMP13 axis-mediated dual modulation of inflammation and cartilage regeneration

doi: 10.1038/s41536-025-00444-9

Figure Lengend Snippet: A Schematic illustration of the ASCs isolation procedure from deer antler tissue using mechanical dissection and the collagenase digestion method. Created in BioRender. Yuhao, S. (2025) https://BioRender.com/m5easfx . B Representative phase-contrast microscopy image showing the typical spindle-shaped morphology of cultured ASCs at passage 3. Scale bar: 100 μm. C Immunofluorescence analysis of ASC surface markers. ASCs were positive for CD73 and CD90 (green), while negative for CD34 and CD45. Nuclei were counterstained with DAPI (blue). Merged images show co-localization. Scale bar: 100 μm. D – F Multi-lineage differentiation potential of ASCs. D Oil Red O staining showing lipid droplets after 14 days of adipogenic induction. Scale bars: 100 μm. E Alizarin Red S staining demonstrating calcium deposition after 21 days of osteogenic differentiation. Scale bars: 500 μm. F Alcian Blue staining revealing proteoglycan synthesis after 14 days of chondrogenic induction. Scale bars: 100 μm. G Western blot analysis confirming the expression of exosomal markers (CD63, TSG101, and CD9) in isolated ASC-Exos compared to ASCs and culture supernatant. H Representative transmission electron microscopy (TEM) image showing the typical cup-shaped morphology of ASC-Exos with characteristic double-membrane structure. Scale bars: 100 nm. I Nanoparticle tracking analysis (NTA) reveals the size distribution of ASC-Exos with an average diameter of 86.4 nm. J Scatter plot from flow cytometry analysis demonstrating the uniform distribution and homogeneity of the isolated ASC-Exos population.

Article Snippet: For flow cytometric analysis, cells were dissociated using trypsin without EDTA, washed with PBS, and incubated with surface marker antibodies at room temperature for 1 h. The panel included positive markers CD73 (bs-4834R, Bioss, China) and CD90 (bs-0778R, Bioss, China), and negative hematopoietic markers CD45 (bs-4819R, Bioss, China) and CD34 (bs-0646R, Bioss, China), following the manufacturer’s recommended antibody concentrations.

Techniques: Isolation, Dissection, Microscopy, Cell Culture, Immunofluorescence, Staining, Western Blot, Expressing, Transmission Assay, Electron Microscopy, Membrane, Flow Cytometry

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel cryoprecipitate-enriched PRP (Cryo-PRP) gel with enhanced mechanical strength and regenerative capacity accelerates enterocutaneous fistula healing

doi: 10.3389/fbioe.2025.1668608

Figure Lengend Snippet: Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of CD34 (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.

Article Snippet: Subsequently, the sections were incubated overnight at 4 °C with rabbit anti-CD34 antibody (BIOSS, bs-064R; 1:100 dilution).

Techniques: Immunofluorescence, Immunohistochemical staining, Staining, Control, Expressing